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In this article, we will discuss about the CRISPR method of molecular biology and forensic sciences. CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated protein 9) is a game-changing genetic engineering tool. It provides a precise and fast method for editing the genomes of many creatures, including humans. Understanding the principles and mechanisms behind CRISPR is critical for comprehending its potential and ramifications.Continue Reading

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In this article, we will discuss about the fascinating applications of PCR. PCR has several applications in medical, clinical, industrial, pharmaceutical and research fields. Sex determination, forensic analysis, mutation detection, pharmacogenomics are some examples of real life applications of polymerase chain reaction. PCR is a technique use to amplify a specific segment of DNA, generating millions of copies from a single DNA molecule. The process involves three main steps: denaturation, annealing, and extension. These steps cycled multiple times to produce exponential amplification of the target DNA.Continue Reading

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In this article, we will discuss about the types polymerase chain reaction. It is a laboratory technique use to amplify and create multiple copies of specific segment of DNA/RNA. There are several types of PCR: inverse PCR, reverse transcriptase PCR, multiplex PCR, etc. We have to move towards the different types PCR due to some demerits of traditional PCR such as non-specific binding and amplification, primer-dimer formation and various other deficiencies.Continue Reading

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In this article, we will discuss about the blotting techniques, their principles and fabulous procedures. Blotting techniques play a pivotal role in molecular biology, enabling scientists to analyze and visualize biomolecules with precision. These techniques have revolutionized the way we study DNA, RNA, and proteins, offering valuable insights into cellular processes. In this article, we will explore the fundamentals of blotting techniques, their applications, and advancements in the field.Continue Reading

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In this article, we will discuss about the enzyme linked immunosorbent assay. It is a biochemical technique use to detect the presence of antigen, antibodies, proteins and other biological molecules in a sample. The principle of ELISA is based the interaction of antigens and antibodies. It used in detection of diseases and pathogens, concentration of antigen and antibodies and several other biological applications.Continue Reading